By Donald Parriott
This advisor for the practising chromatographer who wishes a prepared resource of knowledge on HPLC detection explores and compares present detection platforms and detectors, outlines the typical difficulties linked to a given detector, and provides confirmed ways to averting such difficulties.
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ACKNOWLEDGMENT The author acknowledges the support of LDC Analytical Inc. of Riviera Beach, Florida and, in particular, the support of Douglas A. Perry, who obtained many of the experimental results presented in this chapter. , and Lewin, S. Z. (1960). in "Physical Methods of Organic Chemistry," (A. ), Vol. 1, part 2. Interscience, New York, pp. 1140-1169. , and Wolf, E. (1980). , Pergamon Press, New York, p. 87. "CRC Handbook of Chemistry and Physics," 69th ed. (1988). (R. C. ) CRC Press, Boca Raton, pp.
Change the lamp when it needs it, especially when someone lends you an old detector. Another factor affecting the detector signal-to-noise ratio is the detector time constant. The time constant is controlled by an electrical circuit on the output of the detector amplifier designed to filter out high frequency noise, but to allow slower changes in the detector output, such as the detector response to the elution of a Chromatographie peak, to occur unattenuated. Some manufac turers use a simple R/C (resistor/capacitor) circuit on the detector output, while other vendors use more complex circuits, such as a second-order Bessel filter.
Using a sharp razor blade, cut off the protruding Teflon tubing flush with the Teflon face on the gripper flange. Note that the old Altex plastic tube nut won't work, since the threads don't go far enough down the nut. It was designed for a much thinner flange. Screw the assembly into the flow cell, feeding the tubing through the fairlead on the flow cell housing, and connect the tubing directly to the column with a finger-tight fitting. Finally, it should be noted that peak area measurements are more affected than peak height measurements by overlap from adjacent Chromatographie peaks, as illustrated in Fig.
A Practical Guide to HPLC Detection by Donald Parriott
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